230004 Biological Chemistry

Detaljer
Inst. for Grundvidenskab
Tidligst mulig placering
VarighedEn blok
 
Pointværdi15 (ECTS)
KursustypeKandidatkursus
 
EksamenSluteksamen

skriftlig og mundtlig eksamen


Alle hjælpemidler tilladt

Beskrivelse af eksamen: The oral examination is based on individual presentations of group project reports supplemented with questions to the general pensum.

Vægtning: Oral examination in project report and pensum is judged by 100 %



7-trinsskala, intern censur
 
Forudsætninger for indstilling til eksamenAcceptance of reports of initial exercises.
 
Rammer for undervisningThe course consists of experimental research work based on actual biological issues (approx. 18 h/week) combined with a theoretical part (lectures, colloquy, and theoretical exercises; approx. 3 h/week). Visit to companies using bioprocessing technologies.
 
BlokplaceringBlock 4
Ugestruktur: Outside schedule
 
UndervisningssprogEngelsk
med note om undervisning på dansk
 
Anbefalede forudsætninger230006 Enzymology and Experimental Biochemistry
 
Begrænset deltagerantalThe number of students per course is limited to 30 owing to limited space in the laboratories and the need for intensive teaching in the required research techniques and advanced instrumentations
 
Kompetenceområder
Basic Science:
-Comprehend basic principles required for research studies on bioactive natural products, lipids, dietary fibres, proteins, peptides, enzymes and their inhibitors.
- Transfer the hypothesis through mathematical/statistical description to results, which can be statistical and experimental tested.
- Understanding the theory behind methods in biochemistry with changes in properties of analytes dissolved in water as function of ions, organic molecules and surfactants.
- Evaluate the detection and quantification of experimental data in relation to published values.

Applied Science:
- Expand the knowledge to experimental work with more complex systems as starting materials.
- Knowledge to development of strategy in analytical biological chemistry

Ethics and Values:
- Respond and reflect on environmental related problems in experimental biochemistry.
-Awareness of environmental, health, and disease risks related to bioactive biomolecules and products produced.
 
Kursets målsætning
The aim of the course is to give the students a thorough and fundamental basis for performing experimental research work in the borderline between chemistry, biochemistry, biology and biotechnology. This includes e.g. having broad understanding of the theory behind applied methods and technologies, use of original literature and the ability to transfer ideas from new and traditional methods and technologies to relevant and optimized strategies of experimental procedures and their sequences.
 
Kursusindhold
The course focus on experimental biological chemistry, which especially implies understanding of the relations between structure and properties of the molecules under consideration, in particular biomolecules, which can be macromolecules and/or low molecular weight compounds. Center of attention will be preparative methods usable for isolation of actual native molecules from various types of starting materials and complex matrix systems; this can be of vegetable, animal, or microbial origin, as well as feed or food systems. Strategies and sequences in the required procedures depend on properties of the compounds of interest, the matrix systems and available methods as will be discussed and illustrated in this course.
In some cases, the starting point or part of the course can be de novo synthesis e.g. synthesis of peptides.
A great part of the work in this course will be devoted to investigation of bioactive molecules as e.g. enzymes and their effectors, which give a need for development and use of bioassay.
Following isolation of the actual molecules, an important part of the course will be characterization, identification, and determination of physico-chemical-bioactive properties of the molecules.
In collaborative work (few students per group), the students will use basic and applied research method in the studies of compounds which can be:
- Lipids, oils, lipid soluble vitamins, antioxidants, phytosterols, amphiphilics as phosphor- and glycolipids in membranes.
- Carbohydrates including dietary fibres (plant cell walls) and dietary fibre associated compounds.
- Bioactive peptides, proteins, enzymes and glycoproteins, and cofactors important for their function
- Bioactive natural products and low molecular weight compounds.

The course will give the students basic knowledge to:
- Quality assurance.
- Planning and designing of experimental work.
- Relevant selection of methods and strategies.
- Evaluation and interpretation of produced data and results in relation to literature information.
- Description of obtained results in reports or papers following scientific rules.
 
Undervisningsform
Laboratory work aiming at bringing the students knowledge on how to transfer theory to practical use of the various instruments, biochemical methods, and technologies are central parts of the course. The students will, in groups of three, spend around 18 h per week working with selected research projects. The project will be chosen among a selection of subjects, and agreed on by the students and the supervisor. Main results and conclusions will be presented at a poster session during the final part of the course and the study will be finalized with a project report. Lectures will throughout the course be used to present the theoretical background for general subjects of interest for biological chemistry research implying topics like bioactive natural compounds, structural identification, protein characterization, protein synthesis, and bioinformatics. There will additional be giving an introduction on how to read and interpret original scientific literature. The combined theoretical and practical laboratory experiments with focus on strategies of actual technologies will train the students in calculation and evaluation of produced data, statistical analyses of obtained results, quality assurance and reporting with evaluation of processing effects on product quality based on own data in relation to literature data.
 
Målbeskrivelse
Stipulated in "Areas of Competence the Course Will Address"
 
Litteraturhenvisninger
Depending on the research projects in the course, relevant new and original literature, scientific papers and monographs will be selected.

Examples of supplementary literature can be:
B.W.Wenclawiak, M.Koch, W.Hadjicostas (Eds) (2004: Quality Assurance in Analytical Chemistry. Training and Teachning. Springer Verlag, Berlin, Heidelberg. ISBN 3-540-40578-X.
L.Buckberry and T.Tessdale (2001: Essentials of Biological Chemistry (udvalgte afsnit). John Wiley & Sons Ltd. West Sussex, UK. ISBS 0-471-48906-9.
H.Sørensen, S.Sørensen, C.Bjergegaard, S.Michaelsen (1999): Chromatography and Capillary Electrophoresis in Food Analysis. Royal Society of Chemistry, Cambridge, UK. ISBN 0-85404-561-9.
G.Walsh (2002): Proteins, Biochemistry and Biotechnology, John Wiley and Sons Ltd. West Sussex, UK. ISBN 0-471-899070
D.Voet & J.G.Voet (2004): Biochemistry, John Wiley and Sons, ISBN 0-471-39223-5
 
Kursusansvarlig
Keld Ejdrup Andersen, kea@life.ku.dk, Institut for Grundvidenskab/Biokemi og naturproduktkemi, Tlf: 35332461
 
Studienævn
Studienævn NSN
 
Kursusbeskrivelsesomfang
forelæsninger25
teoretiske øvelser15
praktiske øvelser200
forberedelse160
ekskursioner10
eksamen2

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