240063 Applied Microbiology

Detaljer
Inst. for Jordbrug og Økologi
English titleApplied Microbiology
Tidligst mulig placeringKandidat 1.år til Kandidat 2. år
VarighedEn blok
 
Pointværdi7.5 (ECTS)
KursustypeKandidatkursus
 
EksamenSluteksamen

mundtlig eksamen


Alle hjælpemidler tilladt

Beskrivelse af eksamen: During the course, each group hands in written practicals reports. Examination based on the reports, and on textbook, reviews, and original papers. The students will have a 20 min preparation period before the oral examination.

Vægtning: Reports on practicals 20% Oral examination 80%



7-trinsskala, intern censur
 
Forudsætninger for indstilling til eksamen75% of practical reports submitted
 
Rammer for undervisning
 
BlokplaceringBlock 2
Ugestruktur: A
 
UndervisningssprogEngelsk
 
Begrænset deltagerantalMaximum 24 students
 
Kursusindhold
Teaching will take the form of practical exercises, lectures and colloquia. The following areas will be covered:
* Microbial bioprospecting and population analysis. Detection and genotyping of microorganisms, producers of particular metabolites, and assessment of biodiversity.
*Extremophilic organisms. Strategies for life and metabolic adaptations of cells to extreme conditions. Screening for enzymes and other biologically active molecules. Application of extremophilic organisms.
*Microbial interactions in agriculture. Trophic interactions, plant-microbe interactions, microbiological control of fungal plant pathogens, and importance of microorganisms for soil fertility. Plant-growth promoting rhizobacteria, secondary metabolites with antifungal activities, biosurfactans.
*Aquaculture microbiology: Identification and biology of taste-and-odour producing bacteria in fish ponds and their impact on fish quality.

During the course, the students will obtain hands-on experience with a number of classical and new, cutting edge microbiological techniques, for example: Plate screening, flow cytometry, immunodetection, reporter genes and recombinant bacterial biosensors, Biolog/PLFA/DGGE or T-RFLP fingerprinting, gas chromatography
 
Undervisningsform
Introductory, plenary lectures and seminars/colloquia. Practical laboratory exercises.
 
Målbeskrivelse
The aim of the course is to give the students broad theoretical and practical skills in applied microbiology at an advanced level. In the course, the students will learn classical as well as novel, advanced molecular methods for the characterization of microorganisms and for the study of interactions between microorganisms. Molecular and growth related methods for the characterization of microbial phylogeny will be used. Furthermore, modern gene fusion and fluorescence methods for the study of interactions between microorganisms will be demonstrated. Finally, the students will analyse problems related to ecotoxicology and quality of drinking water. In the course, the students will achieve theoretical as well as practical knowledge on microorganisms related to environmental and biotechnological issues.
The course supplements basic courses at the Bachelor level, e.g. #240044 Miljømikrobiologi, and supports thematic courses at the Masters level.

After completing the course the student should be able to:
Knowledge:
Classify microorganisms phylogenetically
Display overview of extremophilic bacteria and enzymes as well as of their application potential
Describe methods for the investigation of interactions between bacteria and fungi
Display overview of fluorescence based methods for the detection of microorganisms in complex systems and of their viability and metabolism
Describe key issues within ecotoxicology and methods for the detection of bioavailability and toxicity of metals and other pollutants
Display overview of the influence of microorganisms on the quality of drinking water and aquaculture products
Reflect about qualitative and quantitative methods for the detection of the presence of microorganisms in soil and aquatic environments

Skills:
Analyse the phylogenetic relationships of microorganisms and evaluate their potential for production of enzymes and other bioactive molecules.
Analyse the metabolism of microorganisms and their mutual interactions
Evaluate methods for investigation of microorganisms in soil and aquatic environments
Communicate skills on methods for investigation of microorganisms in complex environments

Competences:
Select and discuss basic and advanced methods for investigation of microorganisms in complex environments
Evaluate the mutual interactions between microorganisms and their potential influence on the environment
Evaluate the application potentials of microorganisms within biotechnology and environmental sciences
 
Litteraturhenvisninger
Brock: Biology of Microorganisms, 11th ed. Madigan & Martinko (Prentice Hall). Reviews, original articles, and compendia.
 
Kursusansvarlig
Peter Stougaard, psg@life.ku.dk, Institut for Jordbrug og Økologi/Faggruppe Genetik og Mikrobiologi, Tlf: 35332609
 
Studienævn
Studienævn NSN
 
Kursusbeskrivelsesomfang
forelæsninger32
kollokvier32
praktiske øvelser42
eksamen20
forberedelse80

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