| Responsible Department | Department of Agriculture and Ecology | ||||||||||||||||||||||
| Course Dates | February 2011 | ||||||||||||||||||||||
| Course Abstract | This course aims at communicating advanced knowledge and skills of modern molecular genetic methods and the corresponding statistical techniques in applied plant genetics. We will work with different kinds of DNA markers, consctruction of genetic linkage maps and analysis of genetic diversity in cutivated plants and in natural populations as well as adaptation of plant traits to traditional and new usage profiles. | ||||||||||||||||||||||
| Course Home Page | http://molbreed.life.ku.dk:8080/PhDMarker | ||||||||||||||||||||||
| Course Registration | Use homepage for online registration (http://molbreed.life.ku.dk:8080/PhDMarker) | ||||||||||||||||||||||
| Deadline for Registration | 05-01-2010 | ||||||||||||||||||||||
| Credits | 6 (ECTS) | ||||||||||||||||||||||
| Level of Course | PhD course | ||||||||||||||||||||||
| Language of Instruction | English | ||||||||||||||||||||||
| Restrictions | Max. 16 | ||||||||||||||||||||||
| Course Content | |||||||||||||||||||||||
| . DNA isolation from plant tissues . SSR (single sequence repeats or microsatellites) detection . AFLP (Amplified fragment length polymorphism) detection . Tilling/Eco-tilling (high-throughput method to detect mutations and allelic variations) . Semi-automated fragment analysis on AB (capillaries) and LI-COR (gel) systems. . Sequencing of PCR fragments (AB capillary system) and primer design . SNP (single nucleotide polymorphism) detection . Principles of different marker applications (Diversity, Mapping, QTL detection) | |||||||||||||||||||||||
| Teaching and learning Methods | |||||||||||||||||||||||
| The participants will have two weeks (two times 5 days) of a full-day mixture of theoretical introductions and laboratory work. The main emphasis is on practical experience, the theoretical background targets towards the understanding of the basis of the techniques to enable troubleshooting and the application in the participant's projects. | |||||||||||||||||||||||
| Learning Outcome | |||||||||||||||||||||||
| Even though the course mainly aims to PhD students, senior scientific staff, technical lab staff and other profes-sionals interested in the area are encouraged to consult the course responsible in order to arrange a possible attendance. | |||||||||||||||||||||||
| Course Coordinator | |||||||||||||||||||||||
| Gunter Martin Backes, guba@life.ku.dk, Department of Agriculture and Ecology/Plant and Soil Science, Phone: 353-33434 | |||||||||||||||||||||||
| Other Lecturers | |||||||||||||||||||||||
| Carsten Pedersern, Anna Maria Torp, Jihad Orabi | |||||||||||||||||||||||
| Course Costs | |||||||||||||||||||||||
| The course fee to cover the lab and catering costs for participants from public institutions is 3840 DKK and for participants from the private sector 7680 DKK. | |||||||||||||||||||||||
| Type of Evaluation | |||||||||||||||||||||||
| At the end of the course the participating PhD-students will be asked to give an oral examination on selected papers that are related to the course content. | |||||||||||||||||||||||
| Work Load | |||||||||||||||||||||||
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