240053 Mammalian Genomics

Ansvarligt institutInstitut for Basal Husdyr- og Veterinærvidenskab   90 %
Institut for Plantebiologi og Bioteknologi   5 %
Institut for Jordbrug og Økologi   5 %

English TitleMammalian Genomics
Tidligst mulig placeringBachelor 2. år til Kandidat 2. år
VarighedEn blok
Pointværdi7.5 (ECTS)
Preferentially as BSc course for Biology-Biotechnology students

mundtlig eksamen

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Beskrivelse af eksamen: The 20 min. oral examination is based on the practical laboratory work and on the textbook pages given as examination requirements. The oral exam is divided into 5 min. on a report related subject and 15 min. on a text book related subject. Both subject are drawn at the beginning of the examination, no preparation. The student should show adequate knowledge and understanding of both report and text book related subjects in order to pass the exam.

Vægtning: 100%

7-trinsskala, intern censur
Forudsætninger for indstilling til eksamenAn approved written report is prerequisite for access to oral examination. The oral exam does not include time for preparation, but notes are allowed
Rammer for UndervisningLectures, case work, computer and lab exercies, group presentation. General issues will be taught in common sessions with Plant Genomics, including: bioinformatics, high-throughput sequencing, microRNA and biomarkers.
BlokplaceringBlock 4
Ugestruktur: B
Anbefalede forudsætninger240067 Molekylær genetik
Begrænset deltagerantal30
The following topics will be covered in the theoretical part of the course:
1) Genome projects
2) The mammalian genome
3) Comparative genomics
4) Gene mapping
5) Mapping of disease genes
6) Gene expression
7) Molecular pathology/Cancer/Pharmacogenetics
8) Stydying gene function in the Post-genome Era / Next generation sequencing
9) Genetic testing
10)Genetic manipulation of cells and animals

The case work will focus on the gene that forms the basis for the practical part of the course.

The practical part of the course will contain:
1) DNA sequence analysis (PC-lab)
2) DNA forensics / paternity testing
3) RNA isolation / qPCR (lab work)

More information will be given at the beginning of the course.
Lectures, case work, theoretical and practical exercies, group presentations and group discussions 20% of the lectures are together with Plant Genomics.
Course Objectives
The course is an advanced course in theoretical and practical genomics with focus on mammals. The course will provide information that will allow the student to gain knowledge on methods and technologies currently used to dissect, describe and characterise complex genomes. Aspects of both research and application within the field of genomics will be addressed.

Learning Outcome
After completion of the course the student is expected to have:

Knowledge and understanding
The student should be able to describe the architecture and components of an average mammalian genome. He/she should be able to understand the possibilities of analysing and characterizing mammalian genomes, and to understand qualitative and quantitative genetics with focus on mammalian disease genetics. He/she should be able to describe the technologies and methods involved in genome projects and the objectives of the different genome projects. Furthermore, he/she should understand how comparative genomics can be used in the characterisation of genomes and how comparative genomics can be used to transfer genomic information from one species to another, and understand the regulation of gene expression on different levels. Finally, he/she should understand how mutations can cause inherited defects and predisposition to disease such as cancer and the possibilities and relevance of genetic testing, and know how genomes are annotated.

- Use of basic and advanced methods in molecular genetics.
- Perform DNA sequence analysis, simple sequence assembly and annotation and detect sequence variation by using the UCSC browser.
- Perform PCR
- Perform quantitative PCR and analyse expression profiles of genes in different tissues

Evaluation of strategies to dissect the genetics underlying inherited traits in mammals. Evaluate the practical use of genetic testing.

In order to complete the course with the mark 2, the student should be able to present the aim and the basic principe of the practical part of the drawn subject and present the aim and the basic principle of the practical part of the drawn subject and present the basics on the drawn theoretical subject (listed under Course content).
Human Molecular Genetics. 4th Edition. Tom Strachan, Andrew P. Read. 2011. 781 pages.
Susanna Cirera Salicio, scs@life.ku.dk, Institut for Basal Husdyr- og Veterinærvidenskab/Genetik & Bioinformatik, Tlf: 353-33050
Studienævn NSN
praktiske øvelser30
teoretiske øvelser15