270012 Food Enzymes and Applications

Details
Responsible DepartmentDepartment of Food Science

Earliest Possible YearBSc. 3 year to MSc. 2 year
DurationOne block
 
Credits7.5 (ECTS)
 
Level of CourseJoint BSc and MSc
 
ExaminationFinal Examination

written examination


All aids allowed

Description of Examination: Evaluation of an individual paper based on the project work and related curriculum.

Weight: Written paper: 100 %



7-point scale, internal examiner
 
Requirement for Attending ExamApproval of reports from the practical course.
Completion of project work and oral presentation of the work.
 
Organisation of TeachingLectures 1/3 and exercises: 2/3 per week
 
Block PlacementBlock 3
Week Structure: A
Excersises one day pr. week.

 
Language of InstructionEnglish
 
Restrictions25
 
Course Content
The course will treat the most important food enzymes such as oxidoreductases and hydrolases (proteases, lipases, pectinases, amylases, etc.) with respect to structural and functional properties, catalysis, reaction kinetics and enzyme substrate specificity.
Focus will be on enzyme applications for processing and improvement of foods within the fields of Food Chemistry, Plant Food Science, Dairy Technology and Meat Technology, specifically on the influence of enzymes on chemical and physical changes in foods with respect to preservation of quality and processing of foods.
This comprises two aspects, i.e. 1) enzymes present in foods (milk, meat, fruits and cereals) with major influence on their processing quality, and 2) major exogenous enzymes used in the processsing of these foods into high quality foods and drinks.
 
Teaching and learning Methods
This is a hands-on course and the basic parts will be handled through experimental exercises with various applications of food enzymes practised in small projects. The student will be introduced to several classic enzyme assays and become familiar with quantification of enzyme activity and determination of substrate conversion. The practical exercises will be supported by lectures to provide the necessary theoretical background for the students to carry out the experimental work. Some theoretical exercises and problems will also be touched upon.
 
Learning Outcome
Enzymes are increasingly applied in both food and non-food industries. Advantages obtained by the use of enzymes are the mild reaction conditions, lower risk of toxic by-products, and their great specificity. In food manufacture, enzymes are often applied as processing aids to improve the yield, texture, taste or other quality aspects. The course will be of benefit for students within food technology, biotechnology and related areas.
The main objective of the course is to provide a sound background for the application of enzymes for processing and improvement of foods with focus on the major food groups (dairy, meat and cereal foods and drinks). In addition, the possible advantages and disadvantages of enzymes endogenous in the foods will be emphasised. Major features of enzymes and properties behind their specificity and enzyme assays will be treated theoretically as well as by practical training.

After completion of the course the student should be able to:
Knowledge:
- List the enzyme classes and describe the general effect of enzymes and their regulation
- Describe the mechanism behind the specificity of enzymes
- Describe the major enzymes present in selected foods (milk, meat, wheat and barley) and their influence on the quality of the final products
- describe the mostly used enzymes in the production of selected dairy foods, meat products, bread, beer and food ingredients (glucose syrup, lipids and protein hydrolysates), as well as their function in those products
- provide examples of assays suitable for measurement of various types of enzyme activity and explain the underlying principles

Skills:
- Explain the background for the pH and temperature optimum of enzymes and the nature of co-factors
- Perform laboratory work and handle selected techniques and methods applied in characterisation and use of enzymes
- Design practical work with enzymes, e.g. including use of enzyme assays, and evaluate results obtained
- Apply relevant literature and communicate experiments performed, and results and conclusions obtained within the enzymatic biochemical area in the form of a paper

Competences:
- Cooperate with fellow students about the design, performance and reporting of lab experiments with enzymes and discussion and presentation of obtained results
- Perform and evaluate simple analyses comprising enzymatically catalysed reactions
 
Course Literature
Compendium containing material as mentioned below:

Handbook of Food Enzymology, 2003
ed. John R. Whitaker, Alphons G.J. Voragen & Dominic W.S. Wong
Publisher: Marcel Dekker, Inc.
ISBN: 0-8247-0686-2
selected chapters

Enzymes in Industry. Production and Applications, 3rd edition, 2007
ed. Wolfgang Aehle
Publisher: WILEY-VCH Verlag GmbH & Co. KGaA
ISBN: 978-3-527-31689-2
selected parts

Supplemental papers/chapters
 
Course Coordinator
Jeanette Anita Held Otte, jo@life.ku.dk, Department of Food Science/Food Chemistry, Phone: 353-33189
 
Study Board
Study Committee LSN
 
Work Load
lectures24
practicals93
preparation24
project work61
examination4

206