| Responsible Department | Department of Agriculture and Ecology
45 % Department of Plant Biology and Biotechnology 45 % Department of Basic Animal and Veterinary Sciences 10 % | ||||||||||||||
| Earliest Possible Year | BSc. 2 year to MSc. 2 year | ||||||||||||||
| Duration | One block | ||||||||||||||
| Credits | 7.5 (ECTS) | ||||||||||||||
| Level of Course | Joint BSc and MSc | ||||||||||||||
| Examination | Final Examination written examination Written Exam in Lecturehall All aids allowed Description of Examination: A four hours written examination Weight: 100 % 7-point scale, internal examiner Dates of Exam: 22 June 2012 | ||||||||||||||
| Requirement for Attending Exam | Accepted lab reports (75 %) and oral presentation of case | ||||||||||||||
| Organisation of Teaching | Teaching includes a mix of lectures, laboratory and computer exercises complemented by cases for student groups. Laboratory work demonstrates selected key issues in plant genomics. Computer exercises demonstrate the potential of internet resourcesw | ||||||||||||||
| Block Placement | Block 4 Week Structure: B | ||||||||||||||
| Language of Instruction | English | ||||||||||||||
| Optional Prerequisites | LBIF10184 Molecular Genetics | ||||||||||||||
| Restrictions | None | ||||||||||||||
| Course Content | |||||||||||||||
| * Those contents marked with an asterisk will be offered as shared sessions between the Plant and Mammalian Genomics courses. The Big Picture: Introduction and Overview Crash course, Bioinformatics* and Crash course, Molecular genetics Genomes: Model plants versus crops Next-generation sequencing technologies* Gene expression Transcription factors, focus on MYB family From microarrays to next-generation sequencing (RNA-seq) Forward and reverse genetics Genetic markers and Quantitative Trait Loci Genotype - Phenotype; Map based cloning and QTL dissection Two very different plant genomics models: rice and moss Functional Genomics Metabolomics Biomarkers* and RNAi/siRNA* Student workshop on four functional genomics assignments Functional genomics debate on the findings of the case study In four practical exercises complementing the lectures, you will get familiar with the generation of haploid plant cell cultures, tracking of gene expression, both quantitatively as well as in plant tissue, analysis of quantitative trait loci, and genetic manipulation techniques. | |||||||||||||||
| Teaching and learning Methods | |||||||||||||||
| 50 % for lectures and discussion classes. 50 % for practical exercises and supervised case studies. Students are expected to work in groups for their exercises and cases and written and oral presentations of their results are a requirement for admission to the final exam. | |||||||||||||||
| Learning Outcome | |||||||||||||||
| The course will provide basic understanding of the structure and evolution of plant genomes and central techniques used for studies of genomes and molecular breeding through a combination of lectures, cases, wet-lab exercises and computer exercises. Focus will be on the relationship between phenotypic traits and genotypes using the expanding information and resources on plant genomes and RNA/DNA/protein sequences. The course begins with the genomics and central techniques and databases developed for the two main plant model species, rice and Arabidopsis, and translates the principles to cultivated crops to understand the potential and constraints of applying genomic technology for plant breeding. After completing the course the students should be able to: Knowledge - Describe basic principles for the study of major model plants and general plant evolution. - Explain genetic markers and their use for qualitative and quantitative traits - Compare basic central experimental techniques used in plant genomics and molecular breeding and propose their application for novel challenges Skills - Complete with confidence an assement of the techniques used to study complex biological processes in plant model systems. - Practice molecular and genetic tools for plant improvement through molecular breeding of crops for food, fodder and production of high value crops for e.g. biomedicine, biofuel, green factories. - Manipulate cell cultures and conduct a simp0le transformation procedure. Competences - Evaluate various forward and reverse genomics approaches for gene isolation and functional studies. - Relate gene differences with phenotype by means of genomics - communicate effectively with other students in a debate to justify conclusions on a given problem. | |||||||||||||||
| Course Literature | |||||||||||||||
| Laboratory manuals and a dedicated compendium of selected scientific publications. Next to the compendium, no specific textbook will be required for this course. However, general botany and genetics textbooks may be consulted for the most basic concepts not covered within this course. Plant Genomics is an upper division course; the student should on his or her own initiative located additional reading material that matches the lecture material. | |||||||||||||||
| Course Coordinator | |||||||||||||||
| Søren K. Rasmussen, skr@life.ku.dk, Department of Agriculture and Ecology/Plant and Soil Science, Phone: 353-33436 Björn Robert Hamberger, bjoernh@life.ku.dk, Department of Plant Biology and Biotechnology/Section for Plant Biochemistry, Phone: 353-33328 | |||||||||||||||
| Study Board | |||||||||||||||
| Study Committee NSN | |||||||||||||||
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