LPhD059 Animal Nutrition andPhysiology: Part 1 (theoretical)

Responsible DepartmentDepartment of Basic Animal and Veterinary Sciences   90 %
Department of Veterinary Disease Biology   10 %

Research SchoolAnimal Nutrition and Physiology (RAN)
Course DatesBlock 1, Week Structure: C
Course AbstractThe objective is 1) to provide an introduction to methods used in physiological experimentation and evaluation of nutrient- and energy metabolism in animals. This involves study of physiological and nutritional processes of animal growth, reproduction, lactation and adaptation to different conditions. 2) Fulfil the educational requirements of the EU of persons working with experimental animals. The course includes lectures and practical demonstrations with animals and experimental techniques
Course RegistrationContact course leaders, P. Nørgaard, pen@life.ku.dk or Mette Olaf Nielsen, mon@life.ku.dk
Deadline for RegistrationTwo weeks before start of block 1
Credits7.5 (ECTS)
Level of CoursePhD course
Organisation of TeachingTeaching mainly at Frederiksberg Campus, 3 demonstrations at Rørrendegård Tåstrup (LIFE bus from Frederiksberg Campus to Rørrendegård more times daily)
Language of InstructionEnglish
RestrictionsMaximal 25
Course Content
Teaching and demonstrations are scheduled for 1 1/2 days a week during the first 8 weeks of the course, including weekly tutorials, seminars and demonstrations.
Individual activities will involve reviewing of scientific papers as well as seminar discussions of presented experimental techniques.
In addition students will on individual basis and in groups prepare reports on practical demonstrations based on relevant scientific literature.

The contents of the course covers:
1) Presentation of experimental techniques for assessment of whole animal function and metabolism of nutrients on whole animal and organ level (in vivo/in vitro). This covers both a theoretical introduction as well as practical demonstrations of key techniques.
2) A 30h block which fulfils the educational requirements by EU and Denmark for persons working with experimental animals or assisting in animal experiments (a diploma will be issued upon completion of the course).
3) Principles necessary for planning and implementing in vivo or in vitro scientific investigations as well as for critically evaluating results obtained from such investigations. This includes such aspects as protocol planning, experimental design and data evaluation.
Teaching and learning Methods
Combined lectures, practical demonstrations, seminars/journal clubs and exercises. Students will throughout the course be working in groups Theoretical course with lectures, some laboratory demonstrations and bi-weekly seminars. Practical exercises on live animals. Individual activities will involve reviewing and presentation of handed-out scientific papers as well as seminar discussions of presented experimental techniques, where emphasis will be on evaluation of experimental design, techniques and approaches in relation to aims, obtained results and conclusions.
Learning Outcome
Learning Outcome
- describe the basic principles behind experimental methods for measuring nutritional characteristics of feedstuffs, feed intake, chewing activity, in vivo and in vitro digestibility,
- describe the methods for measuring digestibility in different segments of the digestive tract in monogastic and ruminant animals by use of cannulation and marker techniques, passage rate and digestion kinetics
- describe the theoretical background of energy transfer from cellular to the whole body level, techniques and methods for measuring nitrogen, energy balances, heat production and substrate oxidation
- describe the anatomy and metabolism of splanchnic tissues and catheter techniques used to study tissue and organ nutrient fluxes, practical guidelines for sampling of blood, rumen fluid etc,
- describe the legislation, handling and management of laboratory animals

- be able to describe principles and methods used in selected in vivo demonstrations
- be able to evaluate experimental results/data from different in vivo measurements.
- be able to discus limitations, advantages and their potential applications of the different experimental methods
- be able to interpret experimental results based on theoretical knowledge
- be able to design experimental animal protocols and use relevant legislation for conducting animal experiments

- ability to cooperate with other researches, both inter and intra disciplinary at different levels and with different degree of responsibility
- ability to adopt above described knowledge and skills
- ability to disseminate methodology and obtained results to professionals, lay persons and general public
- ability to perform animal experiments legally according to Council of Europe 1993 resolution category B

The written examination is obligatory in order to obtain EU approved diploma to be allowed working with experimental animals.

In order to pass the practical examination, attendance for the practical experimental animal exercises needs to be at least 80%, and participants not fulfilling this demand cannot take part in the written laboratory animal science examination.

The written exam consists of 16 questions of which up to 5 questions can be multiple choice. Every question gives 0, 1, 2 or 3 points, except multiple choice quesions, which give either 0 or 3 points. To achieve the level 2, a total of at least 24 points must be awarded
Course Literature
Selected reviews/research papers and a list of the literature to be used can be obtained from the course coordinators.

Thomas Krohn: Forsøgsdyrhåndbogen, KU-IVP
Annelise Hem, Dag Marcus Eide, Espen Engh & Adrian Smith: Laboratory Animal Science, Norwegian Veterinary School, 2001, http://oslovet.veths.no/dokument.aspx?dokument=203, chapter 9-14,16-17, 19-26, 28
Course Coordinator
Peder Nørgaard, pen@life.ku.dk, Department of Basic Animal and Veterinary Sciences/Animal Nutrition, Phone: 353-33033
Mette Benedicte Olaf Nielsen, mon@life.ku.dk, Department of Basic Animal and Veterinary Sciences/Animal Nutrition, Phone: 353-33065
Other Lecturers
Christopher Harold Knight, André Chwalibog, Anne-Helene Tauson, Peter Lund, Department of Animal Health, Welfare and Nutrition, Falculty of agricultural Sciences, Aarhus University (DIAS), Niels Bastian Kristensen, DIAS
Course Fee
The course is free of charge for students under the Open Market for Ph.D. courses in Denmark and for students from NOVA-partners. For external students the course fee is 8000 DKK.
Type of Evaluation
One oral examination pass /not pass plus one written examination pass /not pass. Written examination (to obtain EU approved diploma): no aids allowed. Oral examination: all books, reports etc. allowed Description of Examination: Written examination (20 min.) to obtain EU approved diploma for persons working with experimental animals. Weight: It is required to pass the written examinations well as the oral examination.
Work Load
theoretical exercises30
project work0