| Responsible Department | Department of Basic Science and Environment | ||||||||||||
| Course Dates | March 5th 2012 | ||||||||||||
| Course Registration | Send e-mail to jchs@life.ku.dk | ||||||||||||
| Deadline for Registration | February 1st 2012 | ||||||||||||
| Credits | 12 (ECTS) | ||||||||||||
| Level of Course | PhD course | ||||||||||||
| Language of Instruction | English | ||||||||||||
| Course Content | |||||||||||||
| Both course A and B consists of the same theoretical part corresponding to 6 ECTS points. This part will be based on a weekly colloquium or seminars with basis in relevant and selected parts of biochemistry/enzymology textbooks, and original research papers. The experimental part will for course A correspond to 6 ECTS-points and for course B to 12 ECTS points. The basis will be laboratory manuals describing general methods, procedures and techniques, original research papers and the above mentioned theoretical part. The objectives of the course are directed at the opportunities for Ph.D. students to learn more about enzymes, their properties, their effects on metabolic regulations and the various areas of application of enzymes, especially in analytical procedures and bioprocessing technologies. The structure, properties and stability of the enzymes as well as the composition of matrix systems used as enzyme source, will be considered in relation to strategies needed for optimal enzyme isolation. Traditional, new and advanced methods and techniques will be used for the enzyme isolation and characterisation. Optimisation of appropriate assays and consideration of actual inhibitors and activators/cofactors, as well as structure and properties of both substrates and products will be included in the individual projects. The experimental work will be on enzymes, methods and techniques relevant for the individual Ph.D. student projects, or research tasks. | |||||||||||||
| Teaching and learning Methods | |||||||||||||
| Colloquium and seminars, individual study, experimental laboratory project-work and report | |||||||||||||
| Learning Outcome | |||||||||||||
| After completing the course the ph.d. student should be able to: Knowledge: Reflect about physico-chemical-biochemical properties of biomolcules and especially enzymes. Describe strategies to efficient isolation and purification of enzymes from complex biological systems Describe analytical techniques used in biochemistry and for enzyme characterisation. Understand the theoretical basis for changes of water as solvent, extraction and chromatographic systems in work with molecules of different polarity. Skills Evaluate the detection, quantification and linearity of experimental data in relation to published values (literature data). Design experimental stategies in analytical biochemistry and enzymology based on structure and properties of analytes in complex matrix systems. Apply and discuss theoretical principles of analytical biochemistry to carry out experimental isolation and purification of proteins and enzymes. Competences Utilise the obtained knowledge in more comprehensive projects within research, development or industry. | |||||||||||||
| Course Material | |||||||||||||
| The newest editions of books and papers mentioned as basis for teaching arrangements. At present comprising selected parts of: Methods in Enzymology, Advances in Enzymology Biochemistry, 3rd edition, (2004). Donald Voet, Judith G. Voet. Wiley International Edition. ISBN 0-477-39223-5. Chromatogaphy and Capillary Electrophoresis in Food Analysis. Sørensen, H., Sørensen, S., Bjergegaard, C. and Michaelsen, S. The Royal Society of Chemistry, Cambrigde, UK. 470 pp. ISBN 0-85404-561-9. In addition selected original papers corresponding to experimental subjects considered. | |||||||||||||
| Course Coordinator | |||||||||||||
| Jens Christian Sørensen, jchs@life.ku.dk, Department of Basic Sciences and Environment, Phone: 353-32435 | |||||||||||||
| Type of Evaluation | |||||||||||||
| In order to pass the course, a report should be handed in. This and the general theory will be part of the oral exam that allows the ph.d studen to pass | |||||||||||||
| Work Load | |||||||||||||
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| Other Remarks | |||||||||||||
| Colloquium and seminars: 14*3 hours Individual study: 14*7 hours guiding: 10 hours Project-work and report: Module A: 150 hours Module B: 300 hours Total: 300 (modudel A) - 450 hours (Module B) | |||||||||||||