LPhD107 Analytical Chemistry

Responsible DepartmentDepartment of Basic Science and Environment   83 %
Department of Agriculture and Ecology   15 %
Department of Food Science   2 %

Research SchoolEnvironmental Chemistry, Microbiology and Toxicology (RECETO)
Course DatesBlock 3, Week structure C (February-April)
Course RegistrationTo sign up for the course, please contact the PhD office at LIFE-UC or send an e-mail to Jan H Christensen at jch@life.ku.dk.
Deadline for RegistrationAs the course is also a BSc and MSc course the deadline is the same as for ordinary courses in Block 3 at LIFE-UC.
Credits7.5 (ECTS)
Level of CoursePhD course
Language of InstructionEnglish
RestrictionsMax. 50 participants (including BSc and MSc students)
Course Content
The course topics include: An introduction to analytical chemistry: choice of analytical methodology, sampling, sample preparation, chemical analysis, tools for quantitative chemical analysis, quality assurance. Extraction methods such as liquid-liquid extraction, solid phase extraction, super-critical fluid extraction and accelerated solvent extraction. Cleanup and fractionation methods. Introduction to Chromatography, high-pressure liquid chromatography (HPLC), gas chromatography (GC) and other chromatographic methods. Detector types with focus on mass spectrometry and hyphenated techniques such as GC-MS and LC-MS. Introduction to spectroscopic methods (UV-VIS, IR, X-ray, atomic absorption spectroscopy (AAS) and inductive coupled plasma mass spectrometry). Introduction to data processing, errors in chemical analyses, statistical analyses (including chemometrics) and data presentation. Method development, evaluation, validation and QA/QC measures.

Compulsory laboratory exercises comprise projects which involve sampling, extraction and cleanup, methods of analysis, data processing and data evaluation.
Teaching and learning Methods
Lectures and theoretical exercises: The lectures will present the general chemical background of the measurement principles and instrumental techniques as well as theory on sampling, data processing, data analysis, quality assurance and method validation. Theoretical exercises: These exercises will train the students to carry out calculations on the data produced from different instrumental techniques including statistical analyses, reporting and QA/QC. Laboratory exercises: Laboratory exercises represent the heart of the course. Here, the participants train the different instrumental techniques and get used to analytical work in the laboratory. The laboratory exercises comprise 6 compulsory themes. Theoretical and laboratory exercises are performed by groups of 2 or 3 students. A 1-day excursion to a larger accredited laboratory is included in the course.
Learning Outcome
The course objective is to introduce the participants to modern instrumental techniques and analytical approaches within environmental- and soil chemistry, food chemistry and plant biology.

After completing the course the student should be able to:

Describe the theory of sampling, sample preparation and sample preparation techniques
Refer to the chemical theory behind the use of modern instrumental techniques for quantitative chemical analysis
Describe how to identify unknown compounds by mass spectrometry and to measure their concentrations in environmental and food samples

Develop and apply methods for separating chemical compounds in mixtures using chromatography
Perform quantitative chemical analysis of organic compounds and metals
Apply solid data processing and evaluation of analytical data

Develop, validate and apply analytical methods in different field of research
Evaluate and discuss analytical chemical data from the literature.
Do problem solving in analytical chemistry
Course Material
Daniel C. Harris (eight edition): Quantitative Chemical Analysis. Course teachers: Compendium for laboratory exercises, handouts and electronic resources
Course Coordinator
Jan H. Christensen, jch@life.ku.dk, Department of Basic Sciences and Environment, Phone: 353-32456
Other Lecturers
Søren Husted, IJØ Thomas Skov, IFV
Course Fee
There is no course fee for PhD students from Danish universities. All others must pay a fee of DKK 5000
Type of Evaluation
3 hours written examination and 15 - 20 min oral examination of practical aspects of analytical chemistry. The latter is based on the laboratory exercises Vægtning: The written exam 2/3. Oral examination of practical aspects 1/3.
Work Load
theoretical exercises28